Composite

Part:BBa_K1433001

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-09-28)

attB-J23110-attP

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination and mediate DNA inversion from BP state to LR state. Expression of Bxb1 gp35 and gp47 at certain ratio could change DNA from LR state to BP state.

ZJU_int-xis-bplr.gif

Composition:

  1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.
  2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.


This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
    Illegal NheI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 106


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Categories
Parameters
None